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ALBAclone® RhD Grouping Applications


APPLICATION POTENTIAL USAGE
PATIENT TESTING Use two different monoclonal Anti-D antisera Should not detect DVI; if in doubt (eg discrepancies) classify as RhD neg
Recommended Antisera Combinations Cell Line
No 1 Z031 Anti-D alpha LDM1 (IgM)
No 2 Z036 Anti-D beta
 LDM3 (IgM)
OR
No 2 Z041 Anti-D blend LDM3/ESD1 (IgM/IgG)
Epitope Specificity* Comments
6.5
6.5
6.5/9.1 Detects DVI by IAGT only IAGT should not normally be performed on patients
NEONATAL TESTING (See Note 4) Use two different monoclonal Anti-D antisera
Recommended Antisera Combinations Cell Line
No 1 Z031 Anti-D alpha LDM1 (IgM)
No 2 Z041 Anti-D blend LDM3/ESD1 (IgM/IgG)
OR
No1 Z036 Anti-D beta LDM3 (IgM)
No 2 Z039 Anti-D optimum LDM1/ESD1M (IgM/IgM)
Epitope Specificity* Comments
6.5  
6.5/9.1
 
 Will detect all RhD variants including DVI by IAGT only
6.5  
6.5/9.1 Will detect most RhD variants including DVI by direct agglutination
DONOR TESTING Use two different monoclonal Anti-D antisera At least one should detect DVI. If in doubt (eg discrepancies) classify as RhD Pos
Recommended Antisera Combinations Cell Line
No 1 Z036 Anti-D beta LDM3 (IgM)
No 2 Z039 Anti-D optimum LDM1/ESD1M (IgM/IgM)
Epitope Specificity* Comments
6.5  
6.5/9.1
 Will detect most RhD variants including DVI by direct agglutination


Notes
  • These recommendations are based on UKBTS Guidelines and BCSH Guidelines for Compatibility Procedures in Blood Transfusion Laboratories.
  • The detection rate for weak D and RhD variants depends on the technique used. Slide techniques are not recommended for detection of weak D samples.
  • As the cell lines LDM1 and LDM3 have identical RhD epitope specificity, grouping discrepancies will be minimised by using Z031 and Z036 as a matched pair
    (this complies with UKBTS and BCSH guidelines).
  • The latest version of the BCSH Guidelines on Compatibility Procedures (2004) no longer recommends detection of DVI in newborns.