Quant-Rho® FITC anti-D (measurement of Fetomaternal Haemorrhage)
What is the reagent used for?
Estimation of fetomaternal haemorrhage using flow cytometry.
How does this reagent differ from the Kleihauer test (KBT)?
This reagent measures presence of D antigen on red cells whereas the KBT measures haemoglobin F (HbF). Note -this means that the KBT can give a false high result due to the presence of maternal HbF. This will not happen using the Quant-Rho® FITC anti-D.
How is the reagent used?
The reagent is designed for use in flow cytometry, and is ready to use (no dilution needed).
We recommend that a calibration curve, of simulated FMH, is prepared using the procedure described in the IFU. By comparing results from the calibration curve with results from patient samples, the results of the patient bleed volume is determined.
How are the results processed?
The number of positive cell counts is converted to bleed volume using a calculation programme. Details of this calculation can be found in Mollison PL (1972) Quantitation of transplacental haemorrhage - British Medical Journal 3.31-34
How quickly does this reagent give us a result?
The assay, as recommended on the product insert, takes under an hour.
What is the stability?
2 years at 4oC
What is the storage temperature?
4oC - ready to use, no dilution needed
What is the pack size?
5ml
How many tests does the vial contain?
If using 50ul/test, there is sufficient for 100 tests. If using the calibration curve as described on the product insert, then 300ul is required for the curve plus 50ul for every sample. If analysing several samples at the same time, the same calibration curve can be used.
Is the product CE Marked?
Yes, whereas other products on the market available for this purpose may not be.
Is the kit available in the USA?
Yes.
How do I order the product?
Please contact customer services at Alba Bioscience or contact one of our distributors (see website: Enquiries, Distributors for contact details).
Bulk products can also be ordered via customer services.
In the US please contact Alba Bioscience Inc
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